Quantitative analysis of the nuclear landscape
Workflow for quantitative analysis of the nuclear landscape
Volker J Schmid
Professor for Bayesian Imaging and Spatial Statistics
Volker J Schmid ist Professor für Statistik mit Schwerpunkt Bayesianische Statistik für räumliche und Bilddaten.
Publikationen
Distinctive nuclear zone for RAD51-mediated homologous recombinational DNA repair.
Genome-based functions are inseparable from the dynamic higher-order architecture of the cell nucleus. In this context, the repair of …
Cohesin depleted cells pass through mitosis and reconstitute a functional nuclear architecture.
The human genome forms thousands of “contact domains”, which are intervals of enhanced contact frequency. Some, called “loop domains” …
Initial high-resolution microscopic mapping of active and inactive regulatory sequences proves non-random 3D arrangements in chromatin domain clusters
Background: The association of active transcription regulatory elements (TREs) with DNAse I hypersensitivity (DHS[+]) and an open local …
Quantitative analyses of the 3D nuclear landscape recorded with super-resolved fluorescence microscopy
Recent advancements of super-resolved fluorescence microscopy have revolutionized microscopic studies of cells, including the …
Stage-dependent remodeling of the nuclear envelope and lamina during rabbit early embryonic development
Utilizing 3D structured illumination microscopy, we investigated the quality and quantity of nuclear invaginations and the distribution …
Remodeling of the Nuclear Envelope and Lamina during Bovine Preimplantation Development and Its Functional Implications
The present study demonstrates a major remodeling of the nuclear envelope and its underlying lamina during bovine preimplantation …
Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci.
BACKGROUND: A Xist RNA decorated Barr body is the structural hallmark of the compacted inactive X territory in female mammals. Using …
The potential of 3D-FISH and super-resolution structured illumination microscopy for studies of 3D nuclear architecture: 3D structured illumination microscopy of defined chromosomal structures visualized by 3D (immuno)-FISH opens new perspectives for studies of nuclear architecture
Three-dimensional structured illumination microscopy (3D-SIM) has opened up new possibilities to study nuclear architecture at the …