Background: The association of active transcription regulatory elements (TREs) with DNAse I hypersensitivity (DHS[+]) and an open local chromatin configuration has long been known. However, the 3D topography of TREs within the nuclear landscape of …
Recent advancements of super-resolved fluorescence microscopy have revolutionized microscopic studies of cells, including the exceedingly complex structural organization of cell nuclei in space and time. In this paper we describe and discuss tools …
For the statistical analysis of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) data, compartment models are a commonly used tool. By these models, the observed uptake of contrast agent in some tissue over time is linked to physiologic …
Utilizing 3D structured illumination microscopy, we investigated the quality and quantity of nuclear invaginations and the distribution of nuclear pores during rabbit early embryonic development and identified the exact time point of nucleoporin 153 …
The binding behavior of molecules in nuclei of living cells can be studied through the analysis of images from fluorescence recovery after photobleaching experiments. However, there is still a lack of methodology for the statistical evaluation of …
The present study demonstrates a major remodeling of the nuclear envelope and its underlying lamina during bovine preimplantation development. Up to the onset of major embryonic genome activation (MGA) at the 8-cell stage nuclei showed a non-uniform …