FRAP

Bayesian mixed-effects model for the analysis of a series of FRAP images

The binding behavior of molecules in nuclei of living cells can be studied through the analysis of images from fluorescence recovery after photobleaching experiments. However, there is still a lack of methodology for the statistical evaluation of …

Dissection of cell cycle dependent dynamics of Dnmt1 by FRAP and diffusion-coupled modeling

DNA methyltransferase 1 (Dnmt1) reestablishes methylation of hemimethylated CpG sites generated during DNA replication in mammalian cells. Two subdomains, the proliferating cell nuclear antigen (PCNA)-binding domain (PBD) and the targeting sequence …