Thomas Cremer

Latest

• Distinctive nuclear zone for RAD51-mediated homologous recombinational DNA repair.
• Cohesin depleted cells pass through mitosis and reconstitute a functional nuclear architecture.
• Initial high-resolution microscopic mapping of active and inactive regulatory sequences proves non-random 3D arrangements in chromatin domain clusters
• Quantitative analyses of the 3D nuclear landscape recorded with super-resolved fluorescence microscopy
• Remodeling of the Nuclear Envelope and Lamina during Bovine Preimplantation Development and Its Functional Implications
• Reprogramming of fibroblast nuclei in cloned bovine embryos involves major structural remodeling with both striking similarities and differences to nuclear phenotypes of in vitro fertilized embryos
• Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci.
• The potential of 3D-FISH and super-resolution structured illumination microscopy for studies of 3D nuclear architecture: 3D structured illumination microscopy of defined chromosomal structures visualized by 3D (immuno)-FISH opens new perspectives for studies of nuclear architecture
• Double-strand break-induced transcriptional silencing is associated with loss of tri-methylation at H3K4.